Mr Quickie's winning streak was halted at Caulfield as the heavily supported odds-on favourite was run down in the final stride by Danny O'Brien's Grinzinger Star (Reliable Man)
Mr Quickie was aiming for his seventh-straight victory and as a result was forced to carry 6kg more than his rivals, which told at the finish according to apprentice rider Ethan Brown.
"I can't fault his performance and I've got no excuses with the run I had," Brown said.
"He tried hard, he did everything I asked and kicked at the right time, I'm putting it down to the weight he has had to carry.
"He had 6kg more than the rest of the field, I think that was the difference."
Grinzinger Star travelled on the back of Mr Quickie in the run and surged late under the urgings of Linda Meech to win the race by a short half-head.
O'Brien wasn't on course at Caulfield to witness the victory but said Grinzinger Star could be aimed towards the South Australian Derby, with that to be determined following his next run at Caulfield over 2000m in a fortnight.
"I had a good feeling I got there, it didn't lay down the second horse, it had a big weight and mine only had a little weight and I was hoping I was going to get him the whole way down the straight," Meech said.
"I thought I was going to win easier than I did, to the second horse's credit he dug deep.
"Weight stops trains, the other horse had a lot and this horse had none.
"It is nice to ride a winner for Danny, that should keep him happy."
Grizinger Star was bred by The Oaks Stud, from their Group 1 winning mare Artistic, and was sold through their 2017 Yearling Sale draft for NZ$90,000 to Mr JR Wheeler.